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Part:BBa_K812031:Design

Designed by: Chauris Karine   Group: iGEM12_Evry   (2012-09-20)


sfGFP with kozak sequence for eukaryotic expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 19


Design Notes

PCR and cloned in pSB1C3 using EcoRI and PstI


Source

PCR amplified with kozak sequence from DNA coming from Alfonso Jaramillo's lab

References